Enzymatic Sulfation of Mucopolysaccharides in Hen Oviduct
نویسندگان
چکیده
منابع مشابه
Enzymatic Sulfation of Mucopolysaccharides in Hen Oviduct
An enzymatic reaction has been identified in the isthmus of hen oviduct in which sulfate is transferred from 3’-phosphoadenosine 5’-phosphosulfate to mucopolysaccharide acceptors (1). From studies of the reaction with monosaccharides and oligosaccharides as model acceptors (‘2) it was concluded that one or more enzymes in the preparations employed catalyze a direct transfer of sulfate from PAPS...
متن کاملEnzymatic sulfation of mucopolysaccharides in hen oviduct. I. Transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate to mucopolysaccharides.
The oviduct of the laying hen is an organ histologically differentiated into four areas, each with a unique function in the biosynthesis of the egg (2). It contains high concentrations of a number of unusual nucleotides, including uridine diphosphoacetylgalactosamine and uridine diphosphoacetylgalactosamine-(4?)sulfate (3). More recently, it has been observed that uridine diphosphoacetylgalacto...
متن کاملThe enzymatic desulfation of sulfated sugar nucleotides by a novel sulfatase in hen oviduct.
A soluble fraction from the isthmus region of hen oviduct catalyzes the liberation of SOF from UDP-N-acetylgalactosamine-4-sulfate (the nucleotide that accumulates in the same tissue during egg formation). The enzyme is purified some &fold and is shown to catalyze the reactions: (a) UDP-N-acetylgalactosamine-4-sulfate 4 UDP-N-acetylgalactosamine plus SOF; and (b) UDP-N-acetylgalactosamine-4,6-d...
متن کاملImmunoreactive avidin in the hen oviduct mucosa.
diameter)in the gland and small(200 to 700 nm) in the epithelium. Epithelial cells containing secretory granules had a strong resemblance to those of the protodifferentiated gland cells appearing in the magnum of chicks pretreated with hormones. No avidin was observed in any epithelial goblet cells or ciliated cells. The findings paralleled those obtained by biotinylatedenzymes affinity cytoche...
متن کاملGlucocorticoid binding in the hen oviduct.
[(3)H]Triamcinolone acetonide (15nm) was incubated with cytosol (150000g fraction) prepared from oviducts of egg-laying hens. The extent of steroid binding, as determined by charcoal assays, was greatest between 2-4h at 4 degrees C. A similar time curve was obtained when cytosol preparations were first fractionated with (NH(4))(2)SO(4) before labelling. The addition of 10mm-Na(2)MoO(4) or 10mm-...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1960
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)69512-2